Zeiss Axio Observer Z.1

Illumination

• Fluorescence light source (halogen lamp)

Objectives

• EC Plan-neofluar 10x, NA 0.3, WD 5.2 mm
• Plan-apochromat 20x, NA 0.8, WD 0.55 mm
• C-Apochromat 40x, NA 1.3, water immersion, correction collar, WD 0.28 mm
• Plan-apochromat 40x, NA 1.3, oil immersion, WD 0.21 mm
• Plan-apochromat 63x, NA 1.4, oil immersion, WD 0.19 mm
• Plan-apochromat 100x, NA 1.4, oil immersion, WD 0.17 mm

Filter sets

• DAPI ex.: 335 – 383 nm, em.: 420 – 470 nm, DM: 395 nm
• GFP ex.: 450 – 490 nm, em.: 500 – 550 nm, DM: 495 nm
• AF546 ex.: 540 – 552 nm, em.: LP 590 nm, DM: 580 nm
• CY5 ex.: 590 – 650, em.: LP 665, DM 660 nm

Detectors

• Axiocam 305 Color Camera
  • Sensor: SONY IMX 264 Exmor Pregins Global Shutter CMOS (2465 x 2056 px)
  • Pixel size: 3,45 x 3,45 µm
  • Chip size: 8,5 x 7,1 mm
  • Spectral range: 400 – 720 nm
  • Live frame rates: 2464 x 2056 – 36 fps, 1232 x 1028 – 88 fps (2×2 binning)
• Alternative: Hamamatsu ORCA-Flash 4.0 CMOS camera

Stage

• Motorized stage

Software

• ZEN blue control software

Additional equipment

• DIC prism set