Zeiss Axio Observer Z.1 – GAIA

Illumination

Brightfield:

• Halogen lamp

Epifluorescence:

• Fluorescence light source (mercury lamp)

Confocal:

• Solid-state laser 405 nm (20mW)
• Solid-state laser 488 nm (20mW)
• Solid-state laser 561 nm (20mW)
• Solid-state laser 640 nm (20mW)

Objectives

• EC Plan-neofluar 10x, NA 0.3, WD 5.2 mm
• Plan-apochromat 20x, NA 0.8, WD 0.55 mm
• C-Apochromat 40x, NA 1.3, water immersion, correction collar, WD 0.28 mm
• Plan-apochromat 40x, NA 1.3, oil immersion, WD 0.21 mm
• Plan-apochromat 63x, NA 1.4, oil immersion, WD 0.19 mm
• Plan-apochromat 100x, NA 1.4, oil immersion, WD 0.17 mm

Filter sets

Epifluorescence:

• DAPI ex.: 335-383 nm, em.: 420-470 nm, DM: 395 nm
• GFP ex.: 450-490 nm, em.: 500-550 nm, DM: 495 nm
• AF546 ex.: 540-552 nm, em.: LP 590 nm, DM: 580 nm
• CY5 ex.: 590-650, em.: LP 665, DM 660 nm

GAIA confocal unit:

• Band pass filter ET445/58
• Band pass filter ET525/50
• Band pass filter ET600/50
• Band pass filter ET700/75

Detectors

• Axiocam 305 Color Camera – epifluorescence, brighfield
  • Sensor: SONY IMX 264 Exmor Pregins Global Shutter CMOS (2465 x 2056 px)
  • Pixel size: 3.45 x 3.45 µm
  • Chip size: 8.5 x 7.1 mm
  • Spectral range: 400-720 nm
  • Live frame rates: 2464 x 2056 – 36 fps, 1232 x 1028 – 88 fps (2×2 binning)
• Hamamatsu ORCA-Flash 4.0 CMOS camera – confocal, epifluorescence
  • Resolution: 2048 x 2048 px
  • Pixel size: 6.5 µm x 6.5 µm
  • Peak quantum efficiency: 82%
  • Live frame rates: 100 fps at resolution 2048 x 2048 px, binning 2 x 2 and 4 x 4

Scanning module

• GAIA rescanning confocal unit (Confocal.nl)
  • Adjustable pinhole (18 µm, 27 µm, 38 µm, 55 µm, 89 µm)
  • Galvanometer scanner
  • Speed: 3 fps at 512 x 512 px, 30 fps at maximum sprint level at 256 x 256 px

Stage

• Motorized stage

Software

• ZEN blue control software
• MicroManager for GAIA module

Additional equipment

• DIC prism set
• Incubator with adjustable temperature and CO₂ concentration (available upon request)