Olympus SpinSR10

Inverted fluorescence confocal spinning disc microscope with super-resolution mode.

Applications

Fast confocal microscopy with spinning disc technique
Z-stack, time series, positions, mosaic
Super-resolution mode (resolution up to 120 nm)
Advanced automatized experiments

Location

La building, 1.17

Mikroskop Olympus-SpinSR10

Affiliation

„The authors acknowledge the assistance provided by the Research Infrastructure NanoEnviCz, supported by the Ministry of Education, Youth and Sports of the Czech Republic under Project No. LM2018124.“

Specification

Illumination

Halogen lamp for transmission light illumination
CoolLED pE-300ultra fluorescence light source (3x LED: 405, 488, 561 nm)
Solid-state laser 405 nm (50 mW)
Solid-state laser 488 nm (100 mW)
Solid-state laser 561 nm (100 mW)
Solid-state laser 640 nm (100 mW)

Objectives

UPLXAPO 10X, dry, NA 0.4, WD 3.1 mm
UPLXAPO 20X, dry, NA 0.8, WD 0.6 mm
UPLXAPO 40X, dry, NA 0.95, WD 0.18 mm
UPLXAPO 60 X, oil, NA 1.42, WD 0.15 mm

Filter sets

DAPI, ex. 405 (DAPI)
FITC, ex. 488 (GFP)
TRITC, ex. 561 (RFP)
Alexa Fluor 640

Detectors

2x Hamamatsu ORCA-Flash 4.0 CMOS camera for two-channel imaging.
Resolution: 2048 x 2048 pixels
Pixel size: 6.5 µm x 6.5 µm
Peak quantum efficiency: 82 %
Live frame rates: 100 fps at resolution 2048 x 2048 pixels, binning 2 x 2 and 4 x 4

Scanning module

Yokogawa CSU-W1 confocal scanner unit with spinning disk
Pinhole diameter: 50 µm
Tunable rotation speed 1500-4000 rpm
Scan speed >100 fps
Effective view field number 18 mm
Motorized magnification lens for super-resolution mode. XY resolution max 120 nm.

Stage