Olympus FV1200 MPE

Illumination

• Halogen lamp for transmission light illumination
• Fluorescence light source 100W mercury lamp
• Solid-state laser 405 nm (56 mW)
• Solid-state laser 559 nm (100 mW)
• Solid-state laser 635 nm (23 mW)
• Gas laser (Ar) 488/515 nm (22mW)
• Multiphoton laser MaiTai DeepSee 690 – 1040 nm, pulse width <100 fs, avg. power >2.1 W, repetition rate 80 MHz

Objectives

• UPLSAPO10X2 Plan S.Apochromat, NA 0.4, WD 3.1 mm
• UPLSAPO20X U Plan S Apo, NA 0.75, WD 0.6 mm
• UPLSAPO 60X oil, NA 1.35, WD 0.15 mm
• UPLSAPO 100X oil, NA 1.4, WD 0.13 mm
• LUMPLFLN 40X water dipping, NA 0,8, WD 3.3 mm
• LUMPLFLN 60X water dipping, NA 1, WD 2 mm
• XLPLN 25x water dipping/immersion, correction collar, NA 1, WD 4 mm

Filter sets

For fluorescence observation:

• U-MWU2 ex.: 330 – 385 nm, em.: LP 420 nm, DM: 400 nm
• U-MNB2 ex.: 470 – 490 nm, em.: LP 520 nm, DM: 500 nm
• U-MWG2 ex.: 510 – 550 nm, em.: LP 590 nm, DM: 570 nm

Detectors

• 3x confocal detectors (2x Hamamatsu photomultiplier R7862 with spectral detection + 1x
• Hamamatsu photomultiplier R7862 with filter defined detection, single pinhole 50 – 300 µm, 5 µm step)
• 4x detectors for MP excitation imaging (2x GaAsP-PMT + 2x RXD (highly sensitive PMT)Transmitted light detector (Hamamatsu PMT R7400)

Scanning module

• Main scanner for visible lasers (Point optical scanning system with 2 galvo-meter mirrors)
• Secondary SIM scanner for infrared laser (Multi photon simultaneous imaging and laser stimulation)
• Max. resolution: 4096 x 4096 px
• Scanning speed: 2 – 200 µs/px, 9 steps
• Zoom factor: 1 – 50x, 0.5 step

Stage

• PC-controlled motorized stage

Software

• Olympus FluoView control software

Additional equipment

• Okolab microscope incubator with adjustable temperature and CO₂/O₂ concentration, DIC prism set