Olympus FV10i

Illumination

• Laser 405nm (22mW)
• Laser 473nm (15mW)
• Laser 559nm (18mW)
• Laser 635nm (12mW)

Objectives

• UPLSAPO 10x phase contrast objective, NA 0.4, dry
• UPLSAPO 60× phase contrast oil-immersion objective, NA 1.35
• Zoom magnification can be changed continually from 10x to 600x.

Filter sets

• Grating-based emission wavelength selection. Spectral detection system allows to select any wavelength width of the emission spectra in accordance with the used fluorescent dye (no physical filter sets used).

Detectors

• 2x channel confocal PMT detector, 1x Phase contrast channel
• Single motorized pinhole, diameter ø50-800μm with automatic setting

Scanning module

• 2 galvanometer scanning mirrors
• Pixel size: 256 × 256 – 1024 × 1024
• Scanning speed: 1.1 s / frame (for pixel size 512 × 512, High Speed scanning mode)

Stage

• Motorized XYZ stage
• Dedicated specimen holder for glass slides, well slides and glass bottom dish with 35mm diameter

Software

• Olympus FluoView control software
• Map image, one shot, time-lapse (XYT), Z-stack (XYZ), Z-stack time-lapse (XYZT), multi area time-lapse (Multi Area XYT), multi area Z-stack time-lapse (Multi Area XYZT)

Additional equipment

–