LaVision UltraMicroscope II

Upright fluorescence microscope with light-sheet technology for visualization of whole biological samples. Microscope is dedicated to project Neurorecon.

Application

Fast 3D imaging of whole cleared biological samples
Multi-channel, Z-stack, positions, mosaic
Suitable for common clearing media (Cubic, uDIsco)

Location

Lb building, 0.19A

Microscope LaVision-UltraMicroscope-II

Specification

Illumination

Up to 6 light sheets
Solid-state laser 488 nm (50 – 100 mW)
Solid-state laser 561 nm (50 – 100 mW)
Solid-state laser 640 nm (50 – 100 mW)

Objectives

Olympus MVPLAPO 2x, NA 0.5, wd 10 mm.
Additional manual zoom (post-objective) 0.63x – 63x

Filter sets

525/50 (green)
620/60 (red)
680/30 (far-red)

Detectors

sCMOS camera Andor Ixon
5.5 megapixel
Pixel size 6.5×6.5 um
Frame rate up to100 fps

Refractive index matching

1.33 – 1.56

Stage

PC-controlled motorized chamber

Software

Inspector control software, Imaris

Additional equipment

–

Downloads

CellSens manual
Stáhnout PDF
ScanR Acquisition manual
Stáhnout PDF
ScanR Analysis manual
Stáhnout PDF

Contact

Mgr. Barbora Hyklová, Ph.D.

+420 241 062 718

barbora.hyklova@iem.cas.cz

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