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Toxicology and Molecular Epidemiology

Research Department

Group photo of the department staff

The department deals with the study of the toxic effects of nanoparticles and fine particles produced in the air in human cell lines and in 3D lung models (MucilAirTM system). The impact of a polluted environment on humans is evaluated in molecular epidemiological studies. Research also focuses on stem cells and their role in the healing and regeneration of damaged tissues. The methods used include: determination of cytotoxicity, detection of oxidative damage of macromolecules (ELISA), changes in DNA integrity (comet assay, analysis of micronuclei and chromosomal aberrations), gene and protein expression (RT‑PCR, NGS, Western blotting), epigenetic parameters (miRNA, DNA methylation) and DNA repair.

Pavel Rössner

Head of the Department
Pavel Rössner, Ph.D.

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Deputy Head

Michal Šíma, Ph.D.


Pavel Rössner, Ph.D.

Antonín Ambrož, Ph.D.

Hana Barošová, Ph.D.

Barbora Heřmánková, Ph.D.

Prof. Vladimír Holáň, DrSc.

Kateřina Hoňková, Ph.D.

Eliška Javorková, Ph.D.

Zdeněk Krejčík, Ph.D.

Helena Líbalová, Ph.D.

On maternity leave

Assoc. Prof. Stanislav Pavelka

Jitka Pavlíková, Ph.D.

Andrea Rössnerová, Ph.D.

Jitka Sikorová, Ph.D.

Michal Šíma, Ph.D.

Res. Prof. Jan Topinka, Ph.D.

Táňa Závodná, Ph.D.

PhD Students

Tereza Červená, M.Sc.

Kateřina Palacká, M.Sc.

Zuzana Šímová, M.Sc.

Research Assistants and Laboratory Technicians

Fatima Elzeinová, M.Sc.

Irena Chvojková, M.Sc.

Hasmik Margaryan, M.Sc.

Alena Milcová, M.Sc.

Zdeněk Novák, M.Sc.

Zuzana Nováková, M.Sc.

Michaela Sobotková, M.Sc.

Věra Topinková, M.Sc.

Anežka Vimrová, M.Sc.

Kristýna Vrbová, M.Sc.

Important Results

The impact of extractable organic matter from gasoline and alternative fuel emissions on bronchial cell models (BEAS-2B, MucilAir™)

Air pollution caused by road traffic has an unfavorable impact on the environment and also on human health. We focused on extractable organic matter (EOM) from particulate matter from fuels with different ethanol content. We performed cytotoxicity evaluation, quantification of mucin and extracellular reactive oxygen species production, DNA breaks detection, and selected gene deregulation analysis, after one and five days of exposure of human bronchial epithelial model (BEAS-2B) and a 3D model of the human airway (MucilAir™). Our data suggest that the longer exposure had more pronounced effects on the parameters of cytotoxicity and mucin production, while the impacts on ROS generation and DNA integrity were limited.

Schéma specificky deregulovaných genů. Detailní popis naleznete pod obrázkem.

In MucilAir™ no significant differences were detected for E5 between 5-day and 1-day exposure, this EOM caused a differential expression of 13 genes in BEAS-2B cells. The expression of five and eight genes differed after exposure to E20 EOM in MucilAir™ or BEAS-2B cells, respectively, when the 5-day and 1-day treatment was compared.



Šíma, M., Červená, T., Elzeinová, F., Ambrož, A., Beránek, V., Vojtíšek-Lom, M., Kléma, J., Ciganek, M., Rössner, P.: (2022) The impact of extractable organic matter from gasoline and alternative fuel emissions on bronchial cell models (BEAS-2B, MucilAir™). Toxicology in vitro. 80: 105316. doi: 10.1016/j.tiv.2022.105316. Epub 2022 Jan 21. PMID: 35066112.