The Effect of Osteoblast Isolation Methods from Adult Rats on Osteoclastogenesis in Co-Cultures
Cell co-cultures represent the future of in vitro studies important for testing of tissue substitutes and research of diseases. During the development of the co-culture of osteoblasts and osteoclasts, the influence of the osteoblast isolation method on the formation of osteoclasts was investigated. It was found that the explant culture isolation method induces the formation of a higher number of osteoclasts, with a larger area than the explant culture with enzymatic pre-treatment method.
Žižková, R., Hedvičáková, V., Blahnová Hefka, V., Sovková, V., Rampichová, M., Filová, E.: (2022) The Effect of Osteoblast Isolation Methods from Adult Rats on Osteoclastogenesis in Co-Cultures. International Journal of Molecular Sciences. 23(14):7875.