Important Results
The Effect of Osteoblast Isolation Methods from Adult Rats on Osteoclastogenesis in Co-Cultures
Cell co-cultures represent the future of in vitro studies important for testing of tissue substitutes and research of diseases. During the development of the co-culture of osteoblasts and osteoclasts, the influence of the osteoblast isolation method on the formation of osteoclasts was investigated. It was found that the explant culture isolation method induces the formation of a higher number of osteoclasts, with a larger area than the explant culture with enzymatic pre-treatment method.
Rat osteoclasts in a co-culture with osteoblasts isolated with different methods. Number, area, and number of nuclei of rat osteoclasts (rOCs) in a co-culture with osteoblasts isolated by explant culture with enzymatic pre-treatment or by explant culture.
Histochemical staining of co-culture of osteoclasts and osteoblasts isolated with different methods. Histochemical staining of co-culture of osteoclasts and osteoblasts isolated by explant culture with enzymatic pre-treatment or explant culture. White arrows indicate formed osteoclasts. Magnification 40×, scale bar 500 µm; 100×, scale bar 200 µm
Publication:
Žižková, R., Hedvičáková, V., Blahnová Hefka, V., Sovková, V., Rampichová, M., Filová, E.: (2022) The Effect of Osteoblast Isolation Methods from Adult Rats on Osteoclastogenesis in Co-Cultures. International Journal of Molecular Sciences. 23(14):7875.
2023
International Journal of Polymeric Materials and Polymeric Biomaterials. IN PRESS.
