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Tissue Engineering

Research Department

Group photo of the Department of Tissue Engineering team

The department is focused on the development of artificial tissues and we also try to transfer newly developed technologies and know‑how into clinical practice. We are developing the technology of controlled drug delivery from nano/microfiber scaffolds with liposomes for the targeted release of drugs into the defect. We are developing artificial scaffolds for the regeneration of bone and cartilage in clinical practice. We are developing in vitro models of different tissues for toxicological screening of nanoparticals and chemical compounds and for the testing of the effects of antiosteoporotic drugs.

Eva Filová

Head of the Department
Eva Filová, Ph.D.

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People

Deputy Head

Michala Klusáček Rampichová, Ph.D.

Researchers

Eva Filová, Ph.D.

Michala Klusáček Rampichová, Ph.D.

Lucie Wolfová, Ph.D.

Jana Daňková, Ph.D.

On maternity leave

Věra Hedvičáková, Ph.D.

On maternity leave

Věra Sovková, Ph.D.

On maternity leave

Karolína Vocetková, M.D., Ph.D.

On maternity leave

Veronika Hefka Blahnová, Ph.D.

On maternity leave

PhD Students

Barbora Voltrová, M.Sc.

Radmila Žižková, MSc.

Eva Šebová, MSc.

Viktorie Ročková, MSc.

Important Results


The Effect of Osteoblast Isolation Methods from Adult Rats on Osteoclastogenesis in Co-Cultures

Cell co-cultures represent the future of in vitro studies important for testing of tissue substitutes and research of diseases. During the development of the co-culture of osteoblasts and osteoclasts, the influence of the osteoblast isolation method on the formation of osteoclasts was investigated. It was found that the explant culture isolation method induces the formation of a higher number of osteoclasts, with a larger area than the explant culture with enzymatic pre-treatment method.

Rat osteoclasts in a co-culture with osteoblasts isolated with different methods. Number, area, and number of nuclei of rat osteoclasts (rOCs) in a co-culture with osteoblasts isolated by explant culture with enzymatic pre-treatment or by explant culture.

Histochemical staining of co-culture of osteoclasts and osteoblasts isolated with different methods. Histochemical staining of co-culture of osteoclasts and osteoblasts isolated by explant culture with enzymatic pre-treatment or explant culture. White arrows indicate formed osteoclasts. Magnification 40×, scale bar 500 µm; 100×, scale bar 200 µm

 

Publication:

Žižková, R., Hedvičáková, V., Blahnová Hefka, V., Sovková, V., Rampichová, M., Filová, E.: (2022) The Effect of Osteoblast Isolation Methods from Adult Rats on Osteoclastogenesis in Co-Cultures. International Journal of Molecular Sciences. 23(14):7875.


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