Microscopy

Our facility provides training in methods and techniques available on our microscopes. Introductory training is mandatory for every user. Upon request, additional training can be provided in specialized techniques which are not covered by the basic training. These methods include super-resolution microscopy with Airyscan detector, multiphoton microscopy, FRAP (fluorescence recovery after photobleaching), incubation (temperature and atmosphere control), or DIC (differential interference contrast) setting, among others. We also offer consultation and assistance with experimental design or advanced microscope settings.

The facility staff can assist users in selecting the most suitable software for their specific research question and provide training for the software available at the workstations. If needed, direct support with data analysis can be provided, and in specialized cases, custom-made software can be developed on request.

Our facility operates a BSL-2 certified intravital imaging laboratory, which encompasses a spectrum of essential components to facilitate every stage of the experimental process:

Animal handling and housing: Our laboratory is equipped to provide the requisite space and infrastructure for the care and management of small rodents. It can accommodate a diverse range of animals, including transgenic and transfected strains, with the oversight of trained animal caretakers ensuring their welfare.

Surgery room: The surgery room consists of two surgical workstations. It is furnished with a state-of-the-art stereotaxic surgery setup, complete with the necessary equipment for conducting surgical procedures. These include viral injections and cranial window preparations.

In vivo experimental setup: The centerpiece of the laboratory is the 2-photon Femtonics Femto3D Atlas microscope. This advanced microscopy system supports both functional and structural imaging, affording researchers the capacity to investigate biological systems at cellular and subcellular levels. It is particularly suited for in-depth examinations of neural activity, tissue dynamics, and cellular interactions within living organisms. Additionally, the laboratory can accommodate electrophysiological and behavioral experiments.