Location:
Microscopy Service Centre, IEM CAS, Vídeňská 1083
Price:
1000 CZK
Kontaktní osoba
Anotation:
The course will cover theoretical and practical aspects of an „entry-level“ super-resolution imaging methods based on the principle of Optical-Photon-Reassignment available in our facility at confocal microscopes Zeiss LSM880 with AiryScan and Olympus SpinSR10 with OSR.
What is meant by the “entry-level super-resolution microscopy”?
The Entry-Level Super-Resolution microscopy stands for group of methods, which provide resolution improvement by factor of ~2 over the diffraction limited imaging and are very easy to use.
While these methods cannot offer the resolution improvement of factor 5x-10x like STED or STROM/PALM, they are fast, easy to operate and require no special sample preparation or extra skills. They are also very suitable for live cell-imaging.
Main advantages of entry level super-resolution microscopy:
No special sample preparation – same samples as for conventional confocal microscopy, without need of special staining.
One-click super-resolution – Settings and operating is very simple and user-friendly.
Gentle and fast imaging – Methods works with relatively low illumination intensities and short acquisition times for data collection, so they are well suited for live cell imaging.
Limitations:
Only factor of 2
Smaller FOV
Program:
Day 1:
Introduction SR microscopy, explanation of AiryScan and SD-OPR (Spinning Disc Optical Photon Reassignment) principles, facility tour, demonstration of Zeiss LSM 880 AiryScan and Olympus SpinSR10.
Day 2:
Practical hands-on imaging in two parallel groups.
We are open to your own experiments, and your samples are welcome.