Zeiss Axioscan 7

An automated upright slide scanning microscope with brightfield and epifluorescence mode. Suitable for fixed samples on standard glass slides (76 × 26 mm).

Applications

Transmission light microscopy (brightfield, linear and circular polarisation)
Multi-colour fluorescence imaging
Maximum load capacity of 12 slides (76 × 26 mm)
Slide scanning (Tile scan), Stitching, Z-stack, Extended depth of focus (EDF)

Location

Building La, 3rd floor, room 3.1

Zeiss Axioscan 7

Specifications

Illumination

Transmission light microscopy:

White light LED

Fluorescence microscopy:

Fluorescence LED source Viluma 7 FR-R[G/Y]BV-UV

Objectives

5x, dry, 0.15, WD 12.0 mm, N-Achroplan, Pol
10x, dry, 0.25, WD 6.5 mm, N-Achroplan, Pol
10x, dry, 0.45, WD 2 mm, Plan-apochromat
20x, dry, 0.8, WD 0.55 mm, Plan-apochromat
40x, dry, 0.75, WD 0.71 mm, EC Plan-Neofluar
40x, dry, 0.95, WD 0.25 mm, correction collar, Plan-Apochroma

Filter sets

Fluorescence microscopy:

BFP, ex. BP 390/40, em. 450/40, DM 420
GFP, ex. BP 470/40, em. 525/50, DM 495
Cy 3, ex. BP 550/25, em. LP BP 605/70, DM 570
mPlum, ex. BP 587/25, em. BP647/70, DM 605
CY5, ex. BP 640/30, em. BP 690/50, DM 660
Penta band, ex. wavelengths 385, 475, 555, 630 and 735 nm (DAPI, FITC, DsRed, Cy5 and Cy7)

Polarisation microscopy:

Set of filters for circular and linear polarisation (rotation: 0°, 15°, 30°, 45°, 60°, 75°)

Turret capacity: 10 filter cubes

By default, the turret is equipped with fluorescence and circular polarisation filter cubes. Please contact us to exchange these for the linear polarisation filters.

Detectors

Camera Axiocam 705 color R2, Resolution 2464 x 2056 px, Pixel size: 3.45 x 3.45 µm, Spectral range: 400 – 720 nm, Live frame rate 30 frames/s at resolution 1232 x 1028 px (2×2 binning) ·
Hamamatsu ORCA-Flash 4.0 CMOS camera, Resolution: 2048 x 2048 px, Pixel size: 6.5 µm x 6.5 µm, Peak QE: 82%, Live frame rates: 100 frames/s at resolution 2048 x 2048 px, binning 2 x 2 and 4 x 4

Software

ZEN software