Entry Level Super-Resolution Microscopy

Basic Information

The course covers theoretical and practical aspects of an “entry-level” super-resolution imaging methods based on the principle of Optical-Photon-Reassignment available in our facility at confocal microscopes Zeiss LSM880 with AiryScan and Olympus SpinSR10 with OSR.

What is meant by “Entry-Level Super-Resolution Microscopy”? It is a group of methods that improve resolution by a factor of ~2 over diffraction-limited imaging and are very easy to use. While these methods cannot offer the resolution improvement of factor 5x-10x like STED or STROM/PALM, they are fast, easy to operate and require no special sample preparation or extra skills. They are also very suitable for live cell imaging.

• Duration: 3 days
• Language: English
• Location: IEM CAS / Turquoise Auditorium

Programme

Speakers: Štěpán Kortus, Barbora Hyklová, Iva Švecová, Pavel Krist (Zeiss), Dusan Popov (Confocal.nl)

Day 1 / Wednesday / 22.5.2024

Morning session (9:00–11:30, Turquoise Auditorium)

9:00–9:15 – Registration
9:15–9:30 – Microscope service centre of the IEM CAS (Barbora Hyklová)
9:30–10:15 – Introduction to Super-Resolution Microscopy (Štěpán Kortus)
10:00–10:30 – Coffee break
10:30–11:00 – Confocal microscopy for imaging bellow diffraction limit (Barbora Hyklová)
11:00–11:30 – Sample preparation for super-resolution microscopy (Iva Švecová)

11:30–12:30 – Lunch break

Afternoon session (12:30–15:30, Turquoise Auditorium)

12:30–13:15 – Optical-Photon-Reassignment based super-resolution techniques (Štěpán Kortus)
13:15–14:00 – Re-scanning confocal microscopy (Dusan Popov, Confocal.nl)
14:00–14:30 – Coffee break
14:30–15:15 – Super-Resolution with SpinSR10 OSR and AiryScan (Iva Švecová)

Day 2 / Thursday / 23.5.2024 and day 3/ Friday / 24.5.2024

9:00–11:30 – Morning session
11:30–12:30 – Lunch break
12:30–15:00 – Afternoon session

Friday 15:00–15:15 – Course summary (Turquoise Auditorium)

Participants will be divided into 4 groups for following sessions:

• AiryScan hands-on
• SpinSR10 hands-on
• AiryScan data post-processing
• SpinSR10 OSR data post-processing