Zeiss LSM 880 Airyscan

Illumination

• Halogen lamp (HAL 100) for transmission light illumination
• Fluorescence light source 100W mercury lamp
• Solid-state laser 405nm (35mW)
• Ar laser 458nm (25mW)
• Ar laser 488nm (25mW)
• Ar laser 514nm (25mW)
• Solid-state laser 561nm (20mW)
• Solid-state laser 633nm (5mW)

Objectives

• Plan-Apochromat 10X, NA 0.45, WD 2 mm
• Plan-Apochromat 20X, NA 0.8, WD 0.55 mm
• C-Apochromat 40X, NA 1.2, water immersion, correction collar, WD 0.28 mm
• Plan-Apochromat 63X, NA 1.4, oil immersion, WD 0.19 mm
• Alpha Plan-Apochromat 100X, NA 1.46, oil immersion, WD 0.11 mm

Filter sets

• DAPI, ex. G 365, em. BP 445/50, DM 395
• GFP, ex. BP 470/40, em. BP 525/50, DM 495
• RFP, ex. BP 572/25, em. BP 629/62, DM 590
• Cy5, ex. BP 640/30, em. BP 690/50, DM 660
• Triple, BP 420-480 + BP 490-550 + LP650

Detectors

• 2x confocal PMT detector
• 1x 32 channel spectral confocal GaAsP detector
• 1x AiryScan detector
• 1x Transmitted light detector (T-PMT)

Scanning module

• 2 galvanometer scanning mirrors
  • Speed 10 frames/s at resolution 512×512 pixels
  • Resolution up to 8000×8000 pixels

Stage

• PC-controlled motorized stage

Software

• ZEISS ZEN Black Edition for acquisition and processing
  • Modules: AiryScan, FRAP, RICS, tiles
• ZEISS ZEN Blue Edition for processing
  • Modules: AiryScan, 3D visualization, tiles and position, FRAP

Additional equipment

• Temperature and CO₂ controlled incubated enclosure, DIC prism set