PhD Open Position 2024/2025

Development of scaffolds for the regeneration of bones and osteochondral defects

Supervisor:

Head of the Department
Eva Filová, Ph.D.

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Project description

The aim is to develop biodegradable scaffolds which are able to release bioactive substances supporting the formation of new bone or cartilage tissues (e.g. growth factors, peptides, exosomes from stem cells or plant cells), chemicals supporting angiogeneis or drugs (e.g. antibiotics). Foam scaffolds based on collagen, tricalcium phosphates (alpha-TCP, beta-TCP, hydroxyapatite (HA), ceramic scaffolds based on TCP and HA with different nanostructure or microstructure. Moreover, composites with nano/microparticles will be prepared for drug delivery.

Scaffolds will be characterised with physico-chemical methods, SEM etc., seeded with cells – cell lines, stem cells, coculture with other cell types, and tested for cell adhesion, proliferation and differentiation. Selected scaffolds will be tested in vivo (on rats, rabbits, pigs) and evaluated histologically.

Candidate profile

M.Sc. (Mgr.) degree or equivalent in molecular/cellular biology, biochemistry, or medicine; strong interest in cell and molecular biology

Suggested reading

  • Hefka Blahnová V, Vojtová L, Pavliňáková V, Muchová J, Filová E.Int J Mol Sci. 2022 Apr 11;23(8):4236.
  • Filová E, Tonar Z, Lukášová V, Buzgo M, Litvinec A, Rampichová M, Beznoska J, Plencner M, Staffa A, Daňková J, Soural M, Chvojka J, Malečková A, Králíčková M, Amler E.Nanomaterials (Basel). 2020;10(8):1504
  • Zhu H, Blahnová VH, Perale G, Xiao J, Betge F, Boniolo F, Filová E, Lyngstadaas SP, Haugen HJ.Front Cell Dev Biol. 2020 Dec 22;8:619111..
  • The Gradual Release of Alendronate for the Treatment of Critical Bone Defects in Osteoporotic and Control Rats. Hedvičáková V, Žižková R, Buzgo M, Vištejnová L, Klein P, Hovořáková M, Bartoš M, Steklíková K, Luňáčková J, Šebová E, Paurová I, Rysová M, Filová E, Rampichová M. Int J Nanomedicine. 2023 Feb 1;18:541-560.
  • Chang HY, Tuan WH, Lai PL.Mater Sci Eng C Mater Biol Appl. 2021 Jan;118:111421. doi: 10.1016/j.msec.2020.111421.
  • Faruq O, Sayed S, Kim B, Im SB, Lee BT.J Biomed Mater Res B Appl Biomater. 2020 May;108(4):1229-1238

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