11. 05. 2026
μLectures is a monthly seminar series designed to enhance researchers’ understanding of the fundamental workings and principles of microscopy. Each session, lasting 30 minutes, focuses on a specific concept or topic within microscopy. The practical information presented aims to deepen researchers’ comprehension of their microscopy experiments, giving them a better overview of the options available and enabling them to improve their experimental design and more effectively identify and resolve potential issues.
Reproducibility is the breakpoint of transforming fluorescence microscopy from “pretty pictures” into quantitative data. This seminar outlines the practical strategies to minimise experimental variability, focusing on three critical pillars: sample staining, illumination conditions, and image processing. We will look specifically at the challenges of maintaining a constant light dose (irradiation) during long-term experiments, contrasting the requirements for fixed samples with the delicate limits of live-cell imaging. Finally, we will discuss the best practices for reporting acquisition parameters and the processing steps in publications to ensure your data meets modern standards for transparency and verification.
The seminar will take place on Wednesday 3.6.2026 from 2 p.m. (changed from 1 p.m.) in the Turquoise Auditorium of the IEM CAS. It will also be possible to join online using this link (open in a new window).
Registration is not required.
Lecturer: Štěpán Kortus, PhD
