22. 04. 2025
Characterisation of nucleolin protein domains in oocytes and early embryos
Lecturer: Natálie Novotná, M.Sc. / Department of Cell Nucleus Plasticity
Annotation: Nucleolin (NCL) is a multifunctional protein abundantly localised in the nucleolus, mainly known for its functions related to ribosome biogenesis. NCL is essential, and its depletion leads to cellular lethality. But surprisingly, a recent study has described a de novo mutation in NCL in a patient with autism spectrum disorder. This frameshift indel mutation results in the production of a C-terminal deletion form of the protein, which is mislocalised to the nucleoplasm. The observation raises the question of the contribution of individual domains to NCL function and development. To explore this, we generated GFP-tagged truncated mutants to investigate the localisation of NCL domains in oocytes and early embryos.
The seminar will take place on 4. 9. 2025 from 2 p.m. in the Turquoise Auditorium of the IEM CAS.
We look forward to your participation.