09. 04. 2024
The dendrite’s sweet spot: Atypical glycosylation of the neuronal surface and impact on synaptic receptor trafficking, structure and function
Lecturer: Cyril Hanus, PhD. (open in a new window) / The Institute of Psychiatry and Neuroscience of Paris (open in a new window)
The seminar will take place on 30. 04. 2024 from 10:00 a.m. in the Turquoise Auditorium of the IEM CAS.
Annotation:
Like 75% of membrane proteins, neurotransmitter receptors and synaptic adhesion molecules are literally covered by covalently linked sugars, N-glycans. Despite a general awareness that N-glycans may have a strong impact on protein structure and function and are altered in a number of brain pathologies, we know surprisingly little about N-glycosylation in the brain. Our work indicates that a substantial fraction of neuronal surface proteins, notably glutamate AMPA receptors, are locally synthesized in dendrites and use unconventional Golgi-independent pathways to reach the neuronal surface. This local processing modifies protein N-glycosylation states and hence regulates receptor chemical composition and function, unravelling a previously unrecognized mechanism controlling the sensing properties and plasticity of the neuronal membrane.
Keywords: cell-biology, neurotransmitter receptors, synaptic plasticity, cell type-specific gene knockout, electrophysiology, mass spectrometry, molecular dynamics, structural biology