Olympus SpinSR10

Inverted fluorescence confocal spinning disc microscope with super-resolution mode.

Applications

Fast confocal microscopy with spinning disk technique
Multi-channel imaging, Z-stack, time series, positions, mosaic
Super-resolution mode (resolution up to 120 nm)
Advanced automatized experiments
Transmission light microscopy, DIC
Photomanipulation (ablation, uncaging, DNA damage, FRAP)
Total internal reflection fluorescence (TIRF)
uPAINT super-resolution method
Single-particle tracking in 3D
Live-cell imaging

Location

La building, 1.17

Microscope Olympus-SpinSR10

Affiliation

“The authors acknowledge the assistance provided by the Research Infrastructure NanoEnviCz, supported by the Ministry of Education, Youth and Sports of the Czech Republic under Project No. LM2018124.”

Specification

Illumination

Halogen lamp for transmission light illumination
CoolLED pE-300ultra fluorescence light source (3x LED: 405, 488, 561 nm)
Solid-state laser 405 nm (50 mW)
Solid-state laser 488 nm (100 mW)
Solid-state laser 561 nm (100 mW)
Solid-state laser 640 nm (100 mW)

Objectives

UPLXAPO 10X, dry, NA 0.4, WD 3.1 mm
UPLXAPO 20X, dry, NA 0.8, WD 0.6 mm
UPLXAPO 40X, dry, NA 0.95, WD 0.18 mm
UPLXAPO 60X, oil, NA 1.42, WD 0.15 mm
UPLSAPO 60X, water, NA 1.2, WD 0.28 mm

Filter sets

Main dichroic mirror: DM 405/488/561/640
Emission filters for camera 1: B447/60 DAPI, B525/50 FITC, B617/73 TRITC, B685/40
Emission filters for camera 2: B447/60 DAPI, B525/50 FITC
Dual camera splitter: Glass or A561LP

Detectors

2x Hamamatsu ORCA-Fusion BT CMOS camera for two-channel imaging
Resolution: 2304 x 2304 px
Pixel size: 6.5 µm x 6.5 µm
Peak quantum efficiency: 95 %
Live frame rates: 89 fps at resolution 2304 x 2304 px, 100 fps at resolution 2048 x 2048 px, binning 2 x 2 and 4 x 4

Scanning module

Yokogawa CSU-W1 confocal scanner unit with spinning disk
Pinhole diameter: 50 µm
Tunable rotation speed >4000 rpm.,
Scan speed >100 fps
Effective view field number 18 mm
Motorized magnification lens for super-resolution mode. XY resolution max 120 nm.

Stage

PC-controlled motorized stage

Software

CellSens Dimension, ScanR Acquisition, ScanR Analysis

Additional equipment

Photomanipulation module (coming soon)

Andor MicroPoint Galvo Position control Base Unit (scanner)
Equipped with fiberN2 pulse laser with tunable wavelength (dye pump): base wavelength 337 nm, 3 ns, 100 μJ, 20 Hz
Suitable for ablation, uncaging, DNA damage or FRAP

Total internal reflection fluorescence (TIRF) module

TIRF and HiLo illumination with adjustable TIRF angle
Equipped with 640 nm laser (100 mW)
Enables selective illumination of a thin optical section of the sample
Suitable for PAINT super-resolution techniques and high-contrast imaging of structures near the coverslip

Phase mask for single particle tracking in 3D

Double helix phase mask (Spindle, Double Helix Optics)
Introduces z-position dependent PSF distortion to enable precise z-position determination
Suitable for 3D single-particle tracking

Other