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μLectures: Ray optics

μLectures is a monthly seminar series designed to enhance researchers’ understanding of the fundamental workings and principles of microscopy. Each session, lasting 30 minutes, focuses on a specific concept or topic within microscopy. The practical information presented aims to deepen researchers’ comprehension of their microscopy experiments, giving them a better overview of the options available and enabling them to improve their experimental design and more effectively identify and resolve potential issues.

This seminar will focus on the fundamentals of geometrical optics. We will begin by introducing the basic concepts and principles, such as the refractive index and the laws of reflection and refraction of light. Using these principles, we will explain the properties of lenses and then demonstrate how they can be applied to understand the function and design of a microscope. Finally, we will explain how to correctly set up Köhler illumination, which provides ideal and uniform illumination of the entire microscope field of view.

The seminar will take place on Tuesday 31.3.2026 from 1 p.m. in the Turquoise Auditorium of the IEM CAS. It will also be possible to join online using this link (open in a new window). Registration is not required.

Lecturer: Barbora Hyklová, PhD