10. 06. 2025
μLectures is a monthly seminar series designed to enhance researchers’ understanding of microscopy’s fundamental workings and principles. Each session, lasting 30 minutes, focuses on a specific concept or topic within microscopy. The practical information presented aims to deepen researchers’ comprehension of their microscopy experiments, giving them a better overview of the options available and enabling them to improve their experimental design and more effectively identify and resolve potential issues.
This seminar will focus on resolution, point spread function (PSF), and image blur – key concepts that influence the quality and appearance of microscopy images. We will discuss what resolution is, the factors that affect it, and how it relates to the PSF. The PSF describes how a microscope sees a point source of light, such as a single flurophore. Understanding the PSF helps explain the limits of resolution and the impact of various optical aberrations.
We will also briefly talk about super-resolution techniques that rely on the PSF, as well as deconvolution methods that use PSF information to enhance image quality.
The seminar will take place on Tuesday 24.6.2025 from 1 p.m. in the Turquoise Auditorium of the IEM CAS. It will also be possible to join online using this link (open in a new window).
Registration is not required, but we will be happy if you can let us know know if you are planning to come (in person or at iva.svecova@iem.cas.cz).
Lecturer: Iva Švecová
