Institute of Experimental Medicine CAS

About us

Our group provides services and support in field of advanced microscopy for employees of the IEM as well as for the external users. We secure access to the devices, routine service, training, booking system and consultations. Users can access variety of devices from basic fluorescent microscope to the cutting-edge two-photon system for in vivo studies. At this moment, we provide access  to 6 microscopes and one workstation PC via our booking system and we mediate access to another 6 microscopes across the institute. 

 

Devices managed by the Microscopy service centre

Zeiss LSM 880 Airyscan

Super-resolution confocal microscope
Status: Operational

Zeiss LSM 880 Airyscan

Olympus FV1200 MPE

Multiphoton confocal microscope
Status: Operational

Olympus FV1200 MPE

Olympus FV10i

Semi-automatic confocal microscope
Status: Operational

Olympus FV10i

Zeiss AxioObserver Z.1

Epifluorescence microscope
Status: Operational

Zeiss AxioObserver Z.1

Femtonics FEMTO3D Atlas

Fast two-photon fluorescence microscope
Status: Operational

Femtonics FEMTO3D Atlas

Olympus SpinSR10

Super-resolution spinning-disc microscope
Status: Operational

Olympus SpinSR10

DELL Precision 5820

Data analysis & processing workstation
Status: Operational

DELL Precision 5820

 

Other devices offered for sharing

Devices belonging to individual departments which were kindly offered for sharing. For access please contact us.

  • Lightsheet LaVision UltraMicroscope II
  • Zeiss Axio ZOOM.V16
  • Leica DMI6000
  • Olympus IX70
  • LEICA DMRX
  • Philips Morgagni - TEM

 

List of techniques available at our department

  • Transmitted light microscopy
  • Wide field fluorescence microscopy
  • Laser scanning confocal microscopy 
  • Spinning disc confocal microscopy
  • Super-resolution microscopy (Airyscan or SpinSR10)
  • Two-photo excitation
  • 3D ultra-fast laser scanning microscopy with two-photon excitation
  • Fluorescence recovery after photobleaching (FRAP)
  • Light Sheet microscopy

 

Training

We provide training for all our devices. The finished training is requirement for access to the microscopes. See more details here.

 

Consultation/assisted operation

In case of need for help with experiment design, data acquisition or processing please do not hesitate to contact us.

 

Rules for access

Microscopy service center provides access to instrumentation under agreement with determined rules

 

Contacts

Head

Ing. Štěpán Kortus Ph.D.
E-mail: stepan.kortus@iem.cas.cz
Telefon:+420 241 062 725

Stepan Kortus
Deputy head

doc. RNDr. Jan Malínský Ph.D.
E-mail: jan.malinsky@iem.cas.cz
Telefon:+420 241 062 718

Jan Malinsky
Microscopy technician

Mgr. Barbora Hyklová Ph.D.
E-mail: barbora.hyklova@iem.cas.cz
Telefon:+420 241 062 718

Barbora Hyklova