Project description
The aim is to develop biodegradable scaffolds which are able to release bioactive substances supporting healing of chronic wounds. (e.g. growth factors, exosomes from stem cells or plant cells), chemicals supporting angiogenesis or drugs (e.g. antibiotics). Porous foam scaffolds or hydrogels with different nanostructure or microstructure will be combined with nano/microparticles or exosomes to prepare a drug delivery system.
Scaffolds will be seeded with cells – skin cell lines, primary cells, coculture with other cell types, and tested from cell adhesion, migration, proliferation, differentiation and extracellular matrix production. The culture condittions will be adopted to chronic wounds. Selected scaffolds will be tested in vivo (on pigs) and evaluated histologically.
Candidate profile
M.Sc. (Mgr.) degree or equivalent in molecular/cellular biology, biochemistry, or medicine; strong interest in cell and molecular biology
Suggested reading
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- Zhu H, Blahnová VH, Perale G, Xiao J, Betge F, Boniolo F, Filová E, Lyngstadaas SP, Haugen HJ.Front Cell Dev Biol. 2020 Dec 22;8:619111..
- The Gradual Release of Alendronate for the Treatment of Critical Bone Defects in Osteoporotic and Control Rats. Hedvičáková V, Žižková R, Buzgo M, Vištejnová L, Klein P, Hovořáková M, Bartoš M, Steklíková K, Luňáčková J, Šebová E, Paurová I, Rysová M, Filová E, Rampichová M. Int J Nanomedicine. 2023 Feb 1;18:541-560.
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