Department of Tissue Culture and Stem Cells

Department of Tissue Culture and Stem Cells

Department of Tissue Culture and Stem Cells


Head: Assoc. Prof. Pavla Jendelová, Ph.D.

Tel.: +420 241 062 828

The main topics studied in the Department are isolation, labelling and the use of stem cells for the treatment of brain injury, spinal cord and neurodegenerative diseases. Various types of cells (mesenchymal stem cells, neural precursor cell lines derived from fetal spinal cord, or from induced pluripotent cells) are studied, together with anti-inflammatory substances for their potential to promote the regeneration of nervous tissue. Macroporous polymeric hydrogels are used as suitable carriers for cell growth in in vitro cultures as well as for in vivo implantations facilitating the regeneration of the injured tissue. The aim of the cell therapy is to repair, replace or improve biological functions of the damaged neural tissue. For in vivo imaging of grafted cells and drug delivery we utilize magnetic nanoparticles, which are characterized in terms of cytotoxicity and genotoxicity and their influence on grafted cells and host tissue.

Deputy Head:
Lucia Urdzíková-Machová, MD, PhD.
Phone: +420 241 062 619

Research Scientists:
Assoc. Prof. Pavla Jendelová, PhD.
Nataliya Romanyuk, PhD.
Lucia Urdzíková-Machová, MD, PhD.
Aleš Hejčl, MD, PhD.
Klára Jiráková, PhD.
Serhiy Forostyak, MD, PhD.
Karolína Turnovcová, MD, PhD.
Jiří Růžička, PhD

PhD Students:
Dana Mareková, MSc
Kristýna Kárová, MSc
Monika Šeneklová, MSc
Barbora Svobodová, MSc
Petr Krůpa, MD
Miroslava Kapcalová, MSc

Pavlína Macková
Michal Douděra

Important results in 2015

Mesenchymal stem cells reduce the working memory deficit in Alzheimer's disease model.
Stem cell transplantation may have a positive influence and slow the progression of some neurodegenerative diseases. In our study, we transplanted human mesenchymal stem cells (MSCs) into the lateral ventricle of 8 months old transgenic mice (AD-3xTg), which mimic the symptoms of Alzheimer's disease (AD). We studied the changes in the spatial reference and working memory, and the effect of transplanted MSCs on neurogenesis in the subventricular zone (SVZ). We also monitored the levels of harmful oligomer amyloid 56kDa (Ab*56), and the amount of the enzyme glutamine synthetase (GS), which is important for regulating the levels and metabolism of glutamate in the brain, in entorhinal and prefrontal cortex and in the hippocampus, i.e. in the structures that are related with cognitive functions. In 14 months old mice treated with MSC we observed preserved working memory, which may be a result of preserved levels of GS and significantly reduced levels of Ab*56 in the entorhinal cortex (Figure 1). These changes, observed six months after transplantation, were also accompanied by increased cell proliferation in the SVZ. Since the transplanted cells survive in the body of the recipient only for a limited period of time, it is likely that the observed effects could be even more pronounced in case of repeated administration of the stem cells at regular intervals during the life spam of the 3xTg mice.


Working memory test in mice with AD. The red line represents the second trial to find the island in the water maze. The ability to remember the position of the islet is shown at the top left of the chart. AD mice had in the entorhinal cortex reduced level of harmful amyloid oligomer Aβ*56. The graph on the bottom right. AD - Alzheimer's disease, EC - entorhinal cortex, ctrl - control animals were age-matched.



Ruzicka J, Kulijewicz-Nawrot M, Rodrigez-Arellano JJ, Jendelova P, Sykova E. Mesenchymal Stem Cells Preserve Working Memory in the 3xTg-AD Mouse Model of Alzheimer's Disease. Int J Mol Sci. 2016 Jan 25;17(2)

Important results in 2014

1. Neural precursors from induced pluripotent cells (iPS-NP) significantly improve motor function in rats with spinal cord injury.
Grafted cells (iPS-NP) survived well and slowly mature into different neuronal phenotypes (GABAergic, Serotonergic and motoneurons). In addition, they produced growth factors, stimulating neuronal sprouting. As a result, animals with spinal cord injury significantly improved their motor function. Rats were able to support their body weight and perform stepping, so they scored well in tests requiring movement coordination, such as beam walking.


Rats implanted with neural precursors showed better functional outcome after spinal cord ijury and higher volume of spared white and grey matter. Grafted cells robustly survived in the lesion, migrated into the parenchyma and differentiated into gabaergic, dopaminergic and cholinergic neurons.


Collaboration: Brigitte Onteniente, INSERM
Romanyuk N, Amemori T, Turnovcová K, Procházka P, Onteniente B, Syková E, Jendelová P, (2014): Beneficial effect of human induced pluripotent stem cell-derived neural precursors in spinal cord injury repair. Cell Transplant. [Epub ahead of print] IF 3.570.

2. Mesenchymal stem cells increase lifespan of animals with amyotrofic lateral sclerosis (ALS).
We studied the effect of human mesenchymal stem cells (MSCs) in the treatment of an experimental model of ALS. We found that application of MSCs improved motor performance and muscle strength and led to an extended lifespan. MSCs partially rescued motor neuron (MN) loss and decreased apoptosis. MSCs transplantation is therefore a safe procedure able to promote CNS remodeling and regeneration.

The effect of MSC application. After the appearance of fi rst disease symptoms SOD1 rats were treated (arrow) with MSCs (intrathecally, 5×105 cells/50μl) or vehicle-injected (DMEM, 50μl). Shortly after delivery of MSCs disease progression has been slowed down shown by the digger muscle strength (A) and higher motor activity (B). MSC-treated rats lived signifi cantly longer (C, D) and preserved higher number of ventral motor neurons (E) compared with vehicle-injected littermates. We found that SOD1 rats have deteriorated perineuronal nets structure around motor neurons and that application of MSCs partially preserved their structure (F).


Collaboration: Prof. James Fawcett, Cambridge, UK
Forostyak S, Homola A, Turnovcová K, Svítil P, Jendelová P, Syková E, (2014): Intrathecal Delivery of Mesenchymal Stromal Cells Protects the Structure of Altered Perineuronal Nets in SOD1 Rats and Amends the Course of ALS. Stem Cells.;32(12):3163-72. IF 7.133

Important results in 2013

1. Treatment of spinal cord injury using stem cells and biomaterials.
The effect of Neural spinal progenitor cells and/or different types of synthetic hydrogels implanted into acute spinal cord lesion on functional outcome and tissue regeneration was evaluated. Stem cells differentiated into neurons, produced neurotrophins and improved functional outcome., while hydrogels bridged the lesion, facilitate axonal ingrowth into the implant and reduce glial scar formation.
Integration and differentiation of spinal progenitor cells in injured rat spinal cord. Two months after transplantation SPC-01 cells were positive for early transcription factors of interneurons and motor neurons NKX 1.6 and showed an increased expression of mature RNA characters, motorneurons Islet2 and HB9 (A). Four months after the transplantation of the graft, cells were positive for Islet2 and choline acetyl transferase (B) mature markers motoneurons.(Amemori et al., 2013).


Collaboration: King’s College London, Ústav makromolekulární chemie AV ČR
Amemori, T., Romanyuk, N., Jendelová, P., Herynek, V., Turnovcová, K., Procházka, P., Kapcalová, M., Cocks, G., Price, J., Syková, E.: (2013) Human conditionally immortalized neural stem cells improve locomotor function after spinal cord injury in the rat. Stem Cell Res. Ther. 4(3): 68. IF 3,652
Cocks, G., Romanyuk, N., Amemori, T., Jendelová, P., Forostyak, O., Jeffries, A. R., Perfect, L., Thuretm S., Dayanithi, G., Syková, E., Price, J.: (2013) Conditionally immortalized stem cell lines from human spinal cord retain regional identity and generate functional V2a interneurons and motorneurons. Stem Cell Res. Ther. 4(3): 69. IF 3,652
Růžička, J., Romanyuk, N., Hejčl, A., Vetrik, M., Hrubý, M., Cocks, G., Cihlar, J., Přádný, M., Price, J., Syková, E., Jendelová, P.: (2013) Treating spinal cord injury in rats with a combination of human fetal neural stem cells and hydrogels modified with serotonin. Acta Neurobiol. Exp. 73(1): 102-115. IF 1,977.

2. Modification of polymeric hydrogels based on methacrylate, serving to bridge lesions and as carriers for cell therapy.
In cooperation with the Institute of Macromolecular Chemistry ASCR, we developed and tested polymer hydrogels based on methacrylate with different types of pores and surface modified positively charged peptides or RGD sequences. Hydrogels were implanted into a model of acute spinal cord hemisection. The results showed that the positive charge and network pore structure of the RGD sequences on the surface promotes cell growth, both in vitro and in vivo, as well as the ingrowth of axons and blood vessels of the host.

Methacrylate hydrogel with a modified surface bridged the spinal cord lesion (A) and promoted ingrowth of axons (B), blood vessels (C) and astrocytes (D). Served as a suitable carrier for transplanted stem cells.


Collaboration: Ústav makromolekulární chemie AV ČR
Hejčl, A., Růžička, J., Kapcalová, M., Turnovcová, K., Krumbholcová, E., Přádný, M., Michálek, J., Cihlář, J., Jendelová, P., Syková, E.: (2013) Adjusting the chemical and physical properties of hydrogels leads to improved stem cell survival and tissue ingrowth in spinal cord injury reconstruction: a comparative study of 4 methacrylate hydrogels. Stem Cells Dev. 2013 Oct 15;22(20):2794-805. IF 4.670

Intrathecal and intramuscular application of mesenchymal stem cells and their secretome in the treatment of Amyotrophic lateral sclerosis GA ČR 15-06958S Principal Investigator Jendelová, 2015-2017

Biomaterials and stem cells in the treatment of stroke and SCI, MEYS – EEA Norwegian Research Council, 7F14057, 2014-2017

Novel therapeutic approaches to neuronal regeneration following spinal cord injury using functionalized microstructured hydrogels and stem cell, GA ČR 17-11140S, Principal Co-Investigator Jendelova, 2017–2019

Treating Glioblastoma with surface engineered superparamagnetic iron oxide nanoparticles for efficient conjugation with anticancer drugs 17-04918S Principal Investigator Jendelova, 2017–2019

Center of Reconstruction Neuroscience – NEURORECON CZ.02.1.01/0.0/0.0/15_003/0000419 Coordinator Pavla Jendelova 2017–2022


Čejka, Č., Čejková, J., Trošan, P., Zajícová, A., Syková, E., Holáň, V.: (2016) Transfer of mesenchymal stem cells and cyclosporine A on alkali-injured rabbit cornea using nanofiber scaffolds strongly reduces corneal neovascularization and scar formation. Histol. Histopath.,969-980.

Čejková, J., Čejka, Č., Trošan, P., Zajícová, A., Syková, E., Holáň, V.: (2016) Treatment of alkali-injured cornea by cyclosporine A-loaded electrospun nanofibers - An alternative mode of therapy. Exp. Eye Res., 147:128-37.

Forostyak, O., Butenko, O., Anděrová, M., Forostyak, S., Syková, E., Verkhratsky, A., Dayanithi, G.: (2016) Specific profiles of ion channels and ionotropic receptors define adipose- and bone marrow derived stromal cells. Stem Cell Res., 16(3): 622-634.

Forostyak, O., Forostyak, S., Kortus, Š., Syková, E., Verkhratsky, A., Dayanithi, G.: (2016) Physiology of Ca(2+) signalling in stem cells of different origins and differentiation stages. Cell Calcium, 59(2-3): 57-66.

Herynek, V., Turnovcová, K., Veverka, P., Dědourková, T., Žvátora, P., Jendelová, P., Gálisová, A., Kosinová, L., Jiráková, K., Syková, E.: (2016) Using ferromagnetic nanoparticles with low Curie temperature for magnetic resonance imaging-guided thermoablation. Int. J. Nanomed., 11: 3801-3811.

Chudičková, M., Brůža, P., Zajícová, A., Trošan, P., Svobodová, L., Javorková, E., Kubinová, Š., Holáň, V.: (2015) Targeted neural differentiation of murine mesenchymal stem cells by a protocol simulating the inflammatory site of neural injury. J. Tissue Eng. Regen. Med., IN PRESS

Jelínek, M., Bačáková, L., Remsa, J., Kocourek, T., Mikšovský, J., Písařík, P.,Vandrovcová, M., Filová, E.,Kubinová, Š.: (2016) Hybrid Laser Technology for Creation of Doped Biomedical Layers. Journal of Materials Science and Chemical Engineering. 4 (1) 98-104.

Jendelová, P., Kubinová, Š., Sandvig, I., Erceg, S., Sandvig, A., Syková, E.: (2016) Current developments in cell - and biomaterial-based approaches for stroke repair. Expert Opin Biol Ther., 16(1): 43-56.

Jiráková, K., Šeneklová, M., Jirak, D., Turnovcová, K., Vosmanská, M., Babič, M., Horák, D., Veverka, P., Jendelová, P.: (2016) The effect of magnetic nanoparticles on neuronal differentiation of iPS-derived neural precursors Int J Nanomedicine, 6: 6267-6281.

Kaman, O., Dědourková, T., Koktan, J., Kuličková, J., Maryško, M., Veverka, P., Havelek, R., Královec, K., Turnovcová, K., Jendelová, P., Schröfel, A., Svoboda, L.: (2016) Silica-coated manganite and Mn-based ferrite nanoparticles: a comparative study focused on cytotoxicity. J. Nanopart. Res., 18 (4): 100.

Kortus, Š., Srinivasan, C., Forostyak, O., Ueta, Y., Syková, E., Chvátal, A., Zápotocký, M., Verkhratsky, A., Dayanithi, G.: (2016) Physiology of spontaneous [Ca2+]i oscillations in the isolated vasopressin and oxytocin neurones of the rat supraoptic nucleus. Cell Calcium., 59(6): 280-288.

Kortus, Š., Srinivasan, C., Forostyak, O., Zápotocký, M., Ueta, Y., Syková, E., Chvátal, A., Verkhratsky, A., Dayanithi, G.: (2016) Sodium-calcium exchanger and R-type Ca2+ channels mediate spontaneous [Ca2+]i oscillations in magnocellular neurones of the rat supraoptic nucleus. Cell Calcium., 59(6): 289-298.

Lukovic, D., Diez Lloret, A., Stojkovic, P., Rodríguez-Martínez, D., Perez Arago, M.A., Rodriguez-Jimenez F.J., González-Rodríguez, P., López-Barneo, J., Syková, E., Jendelová, P., Kostic, J., Moreno-Manzano, V., Stojkovic,M., Shomi S Bhattacharya, S.S, Erceg S.: (2016) Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions. Stem Cells Transl. Med., IN PRESS

Lukovic, D., Moreno-Manzano, V., Rodriguez-Jimenez, F.J., Vilches, A., Syková,E., Jendelová, P.,Stojkovic, M., Erceg S.: (2016) hiPSC Disease Modeling of Rare Hereditary Cerebellar Ataxias: Opportunities and Future Challenge. Neuroscientist ,IN PRESS

Lunov, O., Zablotskii, V., Churpita, O., Jaeger, A., Polívka, L ., Syková, E., Terebová, N., Kulikov, A., Kubinová, Š., Dejneka, A.: (2016) Towards the understanding of non-thermal air plasma action: effects on bacteria and fibroblasts. RSC Adv., 6(30) 25286-25292.

Lunov, O., Zablotskii, V., Churpita, O., Jäger, A., Polívka, L., Syková, E., Dejneka, A., Kubinová, Š.: (2016) The interplay between biological and physical scenarios of bacterial death induced by non-thermal plasma. Biomaterials., 82: 71-83.

Lunova, M., Zablotskii, V., Dempsey, N.M., Devillers, T., Jirsa, M., Syková, E., Kubinová, Š., Lunov, O., Dejneka, A.: (2016) Modulation of collective cell behaviour by geometrical constraints. Integr. Biol., 8(11): 1099-1110.

Macková, H., Plichta, Z., Proks, V., Kotelnikov, I., Kučka, J., Hlídková, H., Horák, D., Kubinová, Š, Jiráková, K.: (2016) RGDS- and SIKVAVS-Modified Superporous Poly(2-hydroxyethyl methacrylate) Scaffolds for Tissue Engineering Applications. Macromol. Biosci., IN PRESS.

Machová Urdzíková, L., Kárová, K., Růžička, J., Kloudová, A., Shannon, C., Dubišová, J., Murali, R., Kubinová, Š., Syková, E., Jhanwar-Uniyal, M., Jendelová, P.: (2016) The Anti-Inflammatory Compound Curcumin Enhances Locomotor and Sensory Recovery after Spinal Cord Injury in Rats by Immunomodulation. Int. J. Mol. Sci., 17(1).

Novotná, B., Turnovcová, K., Veverka, P., Rössner, P. Jr., Bagryantsevá, Y., Herynek, V., Zvatora, P., Vosmanská, M., Klementová, M., Syková, E., Jendelová, P.: (2016) The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells. Nanotoxicology, 10(6): 662-670.

Růžička, J., Kulijewicz-Nawrot, M., Rodrigez-Arellano, J.J., Jendelová,P., Syková, E.: (2016) Mesenchymal Stem Cells Preserve Working Memory in the 3xTg-AD Mouse Model of Alzheimer's Disease. Int. J. Mol. Sci., 17(2): 152.

Růžička, J., Machová Urdziková, L., Gillick, J., Amemori, T., Romayuk, N., Kárová, K., Závišková, K., Dubišová, J., Kubinová, Š., Murali, R., Syková, E., Jhanwar-Uniyal, M., Jendelová, P.: (2016) A comparative study of three different types of stem cells for treatment of rat spinal cord injury. Cell Transplant., IN PRESS

Syková, E., Rychmach, P., Drahorádová, I., Konrádová, Š., Růžičková, K., Voříšek, I., Forostyak, S., Homola, A., Bojar, M.: (2016) Transplantation of mesenchymal stromal cells in patients with amyotrophic lateral sclerosis: Results of Phase I/IIa clinical trial. Cell Transplant., IN PRESS

Školoudik, L., Chrobok, V., Kalfert, D., Koči, Z., Syková, E., Chumak, T., Popelář, J., Syka. J., Laco, J., Dědková, J., Dayanithi, G., Filip, S.: (2016) Human multipotent mesenchymal stromal cells in the treatment of postoperative temporal bone defect: an animal model. Cell Transplant., 25(7): 1405-1414.

Šponer, P., Filip, S., Kučera, T., Brtková, J., Urban,K., Palička, V., Kočí, Z., Syka, M., Bezrouk, A., Syková, E.: (2016) Utilizing Autologous Multipotent Mesenchymal Stromal Cells and -Tricalcium Phosphate Scaffold in Human Bone Defects: A Prospective, Controlled Feasibility Trial. Biomed Res. Int., 2076061.

Tukmachev, D., Forostyak, S., Kočí, Z., Závišková, K., Vacková, I., Výborný, K., Sandvig, I., Sandvig, A., Medberry, C.J., Badylak, S.F., Syková, E., Kubinová, Š.: (2016) Injectable Extracellular Matrix Hydrogels as Scaffolds for Spinal Cord Injury Repair. Tissue Eng., 22(3-4): 306-317.

Voříšek, I., Syka, M., Vargová, L.: (2016) Brain Diffusivity and Structural Changes in the R6/2 Mouse Model of Huntington Disease. J. Neurosci. Res., IN PRESS.

Zablotskii, V., Lunov, O., Kubinová, Š., Polyaková, T., Syková, E., Dejneka, A.: (2016) Effects of high-gradient magnetic fields on living cell machinery. J. Phys. D-Appl. Phys., 49: 493003.



Amemori, T., Jendelová, P., Růžička, J., Urdziková, L.M., Syková, E.: (2015) Alzheimer's Disease: Mechanism and Approach to Cell Therapy. Int J Mol Sci. 16(11): 26417-26451.

Amemori, T., Růžička, J., Romanyuk, N., Jhanwar-Uniyal, M., Syková, E., Jendelová, P.: (2015) Comparison of intraspinal and intrathecal implantation of induced pluripotent stem cell-derived neural precursors for the treatment of spinal cord injury in rats. Stem Cell Res Ther. 6(1): 257.

Babič, M., Schmiedtová, M., Poledne, R., Herynek V., Horák D.: (2015) In vivo monitoring of rat macrophages labeled with poly(l-lysine)-iron oxide nanoparticles. J Biomed Mater Res B Appl Biomater. 103(6): 1141-1148.

Forostyak, O., Romanyuk, N., Verkhratsky, A., Syková, E., Dayanithi G.: (2015) Plasticity of calcium signaling cascades in human embryonic stem cell-derived neural precursors. Stem Cells Dev., 22(10): 1506-1521.

Havlas, V., Kotaška, J., Koníček, P., Trč, T., Konrádová, Š., Kočí, Z., Syková, E.: (2015) Use of cultured human autologous bone marrow stem cells in repair of a rotator cuff tear: preliminary results of a safety study. Acta Chir Orthop Traumatol Cech. 82(3): 229-234.

Hejtčl, A., Jendelová, P., Sameš, M., Syková, E.: (2015) Experimental Treatment of Spinal Cord Injuries. Česká A Slovenska neurologie a neurochirurgie 78(4): 377-392.

Jelínek, M., Kocourek, T., Zemek, J., Mikšovský, J., Kubinová, Š., Remsa, J., Kopeček, J., Jurek, K.: (2015) Chromium-doped DLC for implants prepared by laser-magnetron deposition. Mater. Sci. Eng. C-Mater. Biol. Appl. 46: 381-386.

Klíma, K., Vaněček, V., Kohout, A., Jiroušek, O., Foltán, R., Stulík, J., Machoň, V., Pavlíková, G., Jendelová, P., Syková, E., Šedý, J.: (2015) Stem cells regenerative properties on new rat spinal fusion model. Physiol. Res. 64(1): 119-128.

Kubinová, Š., Horák, D., Hejčl, A., Plichta, Z., Kotek, J., Proks, V., Forostyak, S., Syková, E.: (2015) SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores for spinal cord injury repair. J Tissue Eng Regen Med. 9(11): 1298-1309.

Kwiecien, J. M., Jarosz, B., Machová-Urdziková, L., Rola, R., Dabrowski, W.: (2015) Subdural infusion of dexamethasone inhibits leukomyelitis after acute spinal cord injury in a rat model. Folia Neuropathol., 64(1): 41-45.

Lukovic, D., Moreno-Manzano, V., Lopez-Mocholi, E., Rodriguez-Jiménez, F.J., Jendelová, P., Syková, E., Oria, M., Stojkovic, M., Erceg, S.: (2015) Complete rat spinal cord transection as a faithful model of spinal cord injury for translational cell transplantation. Sci Rep., 5: 19640.

Lukovic, D., Stojkovic, M., Moreno-Manzano, V., Jendelová, P., Syková, E., Bhattacharya, S. S., Erceg S.: (2015) Reactive astrocytes and stem cells in spinal cord injury: good guys or bad guys?Stem Cells. 33(4) :1036-1041.

Lunov, O., Churpita, O., Zablotskii, V., Deyneka, I.G., Meshkovskii, I.K., Jäger, A., Syková, E., Kubinová,Š., Dejneka, A.: (2015) Non-thermal plasma mills bacteria: Scanning electron microscopy observations. Applied Physics Letters. 106 (5) : 053703.

Raha-Chowdhury, R., Raha, A.A., Forostyak, S., Zhao, J.W., Stott, S.R., Bomford, A.: (2015) Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neurosci., 16: 24.

Romanyuk, N., Amemori, T., Turnovcová, K., Procházka, P., Onteniente, B., Syková, E., Jendelová, P.: (2015) Beneficial effect of human induced pluripotent stem cell-derived neural precursors in spinal cord injury repair.Cell Transplant.24(9): 1781-1797.

Syka, M., Keller, J., Klempíř, J., Rulseh, A.M., Roth, J., Jech, R., Vořišek, I., Vymazal, J.: (2015) Correlation between relaxometry and diffusion tensor imaging in the globus pallidus of Huntington's disease patients. PLoS One., 10(3): e0118907.

Tukmachev, D., Lunov, O., Zablotskii, V., Dejneka, A., Babič, M., Syková, E., Kubinová, Š.: (2015) An effective strategy of magnetic stem cell delivery for spinal cord injury therapy. Nanoscale.7(9): 3954-3958.

Institute for Clinical and Experimental Medicine

Institute of macromolecular chemistry ASCR

Physical Institute ASCR

2nd Faculty of Medicine, Charles University

Faculty of Medicine Hradec Králové, Charles University

Faculty of Biomedical Engineering, Czech Technical University