IEM head

Department

Department of Cellular Neurophysiology

Head

Miroslava Anděrová, PhD

Website

http://www.iem.cas.cz/en/research/departments/department-of-cellular-neurophysiology/

Project title

The role of TRPV4 and AQP4 channels in ischemic brain injury and subsequent regeneration

Supervisor

Miroslava Anděrová, PhD

Project description

TRPV4 nonselective cation channels are members of the TRP (transient receptor potential) channel superfamily, and are broadly expressed throughout the nervous system in neurons and several glia subpopulations, including astrocytes, NG2 glia and microglia. Due to this broad expression and their different methods of activation, TRPV4 channels are involved in numerous physiological and pathological processes. They are also suspected of cooperation with other channels found on cellular membranes, such as Aquaporin 4 (AQP4). The aim of the project will be to characterize cell-type-specific roles of TRPV4 and AQP4 channels in pathological processes, and to evaluate the influence of these channels on ischemic brain injury and regenerative processes. To achieve these aims, laboratory techniques, such as immunofluorescence, patch-clamp, fluorescence-activated cell sorting, and RNA-Seq analysis will be performed on specimens isolated from control and conditional Trpv4 and AQP4 knockout mice.

Candidate profile

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